Author: Su, Xiaoping; Qian, Cheng; Zhang, Qian; Hou, Jin; Gu, Yan; Han, Yanmei; Chen, Yongjian; Jiang, Minghong; Cao, Xuetao
Title: miRNomes of haematopoietic stem cells and dendritic cells identify miR-30b as a regulator of Notch1 Document date: 2013_12_6
ID: 4vo7n6nh_29
Snippet: Preparation of bone marrow-derived DC subsets. Mouse imDCs were prepared from bone marrow progenitors by culturing in 10 ng ml À 1 rmGM-CSF and 1 ng ml À 1 rmIL-4. Nonadherent cells were gently washed out on the third day of culture; the remaining loosely adherent clusters were used on day 6 as imDCs. DCreg derived from maDCs were generated by co-culturing with splenic stroma 15 ......
Document: Preparation of bone marrow-derived DC subsets. Mouse imDCs were prepared from bone marrow progenitors by culturing in 10 ng ml À 1 rmGM-CSF and 1 ng ml À 1 rmIL-4. Nonadherent cells were gently washed out on the third day of culture; the remaining loosely adherent clusters were used on day 6 as imDCs. DCreg derived from maDCs were generated by co-culturing with splenic stroma 15 .
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