Author: Jennifer A. Doudna
Title: Blueprint for a Pop-up SARS-CoV-2 Testing Lab Document date: 2020_4_12
ID: modtthxx_104
Snippet: A serial dilution of the SARS-CoV-2 positive control RNA in the ThermoFisher TaqPath COVID-19 Control Kit was prepared in our sample collection medium (2X DNA/RNA Shield diluted to 1X in phosphate-buffered saline (PBS)), spanning 1 x 10 2 to 5 x 10 4 genomic copies of SARS-CoV-2 RNA per ml. The RT-qPCR reaction was run using triplicates at each concentration. The threshold values were set manually based on background fluorescence of the reaction .....
Document: A serial dilution of the SARS-CoV-2 positive control RNA in the ThermoFisher TaqPath COVID-19 Control Kit was prepared in our sample collection medium (2X DNA/RNA Shield diluted to 1X in phosphate-buffered saline (PBS)), spanning 1 x 10 2 to 5 x 10 4 genomic copies of SARS-CoV-2 RNA per ml. The RT-qPCR reaction was run using triplicates at each concentration. The threshold values were set manually based on background fluorescence of the reaction mixture. MS2 bacteriophage genomic RNA (Thermo Fisher) was used as an internal control for RNA extraction, reverse transcription, and PCR amplification. We then performed 20 replicates at our target LoD to confirm the limit of detection. CC-BY-ND 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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