Selected article for: "master mix and Thermo Scientific master mix"
Author: Lyoo, Kwang-Soo; Yeom, Minjoo; Kim, Jungho; Kim, Donghyuk; Ha, Gunwoo; Na, Woonsung; Le, Van Phan; Song, Daesub
Title: Development of rapid immunochromatographic strip test for the detection of porcine epidemic diarrhoea virus
  • Document date: 2017_12_2
    • ID: 4szmu1dh_12
    Snippet: [FAM]-5'-TGT TGCC ATTG CCAC GACTCCTGC-3'-BHQ1), 4x TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) and water. The reaction was performed on a LightCycler 480 System (Roche Diagnostics, Basel, Switzerland) beginning with reverse transcription at 58°C for 30 minutes, followed by heat denaturation at 95°C for 5 minutes, 40 cycles of denaturation at 95°C for 30 seconds, and annealing and extension at 60°C for 1 minute. The data wer.....
    Document: [FAM]-5'-TGT TGCC ATTG CCAC GACTCCTGC-3'-BHQ1), 4x TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) and water. The reaction was performed on a LightCycler 480 System (Roche Diagnostics, Basel, Switzerland) beginning with reverse transcription at 58°C for 30 minutes, followed by heat denaturation at 95°C for 5 minutes, 40 cycles of denaturation at 95°C for 30 seconds, and annealing and extension at 60°C for 1 minute. The data were analysed using LC480 software (Roche Diagnostics). Serial tenfold dilutions of the PEDV-DR13 strain including TEGV, PRCV and porcine rotavirus corresponding 1×10 6.0 to 1×10 1.0 TCID 50 /ml were analysed with the ICA kit and real-time RT-PCR.

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