Selected article for: "chain reaction and PCR quantitative polymerase chain reaction"
Author: Leung, Ho-Chuen; Chan, Chris Chung-Sing; Poon, Vincent Kwok-Man; Zhao, Han-Jun; Cheung, Chung-Yan; Ng, Fai; Huang, Jian-Dong; Zheng, Bo-Jian
Title: An H5N1-based matrix protein 2 ectodomain tetrameric peptide vaccine provides cross-protection against lethal infection with H7N9 influenza virus
  • Document date: 2015_4_8
    • ID: 14qckds8_11
    Snippet: Viral RNA copies in mouse lung homogenates were measured by quantitative reverse-transcription polymerase chain reaction (QRT-PCR) as described previously. 23 Briefly, viral RNA of mouse lung homogenate was extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The extracted viral RNA was reverse-transcribed to cDNA using Superscript II (Invitrogen, Waltham, MA, USA) and primer Flu12 (AGC AAA AGC). The viral RNA copy numbers in the samp.....
    Document: Viral RNA copies in mouse lung homogenates were measured by quantitative reverse-transcription polymerase chain reaction (QRT-PCR) as described previously. 23 Briefly, viral RNA of mouse lung homogenate was extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). The extracted viral RNA was reverse-transcribed to cDNA using Superscript II (Invitrogen, Waltham, MA, USA) and primer Flu12 (AGC AAA AGC). The viral RNA copy numbers in the samples were determined using a Lightcycler 96 (Roche Applied science, Penzberg, Germany) with SYBR green I master (Roche Applied science, Penzberg, Germany). Primers specific for influenza viral M gene (forward primer: 5-CTT CTA ACC GAG GTC GAA ACG-3; reverse primer: 5-GGC ATT TTG GAC AAA KCG TCT A-3) were used in the QRT-PCR assay.

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