Selected article for: "capture probe and nucleic acid"
Author: Yu, Xiaobo; Song, Lusheng; Petritis, Brianne; Bian, Xiaofang; Wang, Haoyu; Viloria, Jennifer; Park, Jin; Bui, Hoang; Li, Han; Wang, Jie; Liu, Lei; Yang, Liuhui; Duan, Hu; McMurray, David N.; Achkar, Jacqueline M.; Magee, Mitch; Qiu, Ji; LaBaer, Joshua
Title: Multiplexed Nucleic Acid Programmable Protein Arrays
  • Document date: 2017_9_20
    • ID: 7t1o19kn_3
    Snippet: A primary disadvantage of planar-based cell-free protein microarrays is the diffusion of mRNA or expressed proteins during in vitro transcription and translation (IVTT), which can then be captured by neighboring features (i.e., cross-talk). Thus, the closer the features are to each other, the higher the cross-talk [21] [22] [23] [24] . Planar-based cell-free protein microarrays include the protein in situ array (PISA) [22] , DNA array to protein .....
    Document: A primary disadvantage of planar-based cell-free protein microarrays is the diffusion of mRNA or expressed proteins during in vitro transcription and translation (IVTT), which can then be captured by neighboring features (i.e., cross-talk). Thus, the closer the features are to each other, the higher the cross-talk [21] [22] [23] [24] . Planar-based cell-free protein microarrays include the protein in situ array (PISA) [22] , DNA array to protein array (DAPA) [25] , nucleic acid programmable protein array (NAPPA) [18, [26] [27] [28] , and in situ puromycin-capture array [29] . DAPA, NAPPA and puromycin-capture arrays employ a probe (e.g., Ni-NTA or anti-tag antibody) on a microarray surface that captures the expressed recombinant proteins in situ during IVTT.

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