Selected article for: "digested plasmid and dna fragment"
Author: Li, Yuetao; Zhao, Yongkun; Wang, Cuiling; Zheng, Xuexing; Wang, Hualei; Gai, Weiwei; Jin, Hongli; Yan, Feihu; Qiu, Boning; Gao, Yuwei; Li, Nan; Yang, Songtao; Xia, Xianzhu
Title: Packaging of Rift Valley fever virus pseudoviruses and establishment of a neutralization assay method
  • Document date: 2018_3_23
    • ID: 4nphwznx_7
    Snippet: The pUC57-Simple-M recombinant plasmid was used as the template, and polymerase chain reaction (PCR) amplification was performed with the synthesized primers. The obtained DNA fragment was subjected to double digestion and was then ligated into a double-digested eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was confirmed to have the correct ligation by gene sequencing performed by Beijing Genomics Institute (China). Finally, t.....
    Document: The pUC57-Simple-M recombinant plasmid was used as the template, and polymerase chain reaction (PCR) amplification was performed with the synthesized primers. The obtained DNA fragment was subjected to double digestion and was then ligated into a double-digested eukaryotic expression plasmid (pcDNA3.1). The recombinant plasmid was confirmed to have the correct ligation by gene sequencing performed by Beijing Genomics Institute (China). Finally, the recombinant expression plasmid was extracted for future use and named pcDNA3.1-M-rvfv.

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