Title: Targeting of protein ERGIC-53 to the ER/ERGIC/cis-Golgi recycling pathway Document date: 1995_10_1
ID: 7oklz2ch_9
Snippet: For further mutagenesis, GM was cloned as a ClaI/XbaI fragment into AccI/XbaI cut pECE. The resulting plasmid was designated pECE*. This cloning step eliminated the single AccI site in pECE so that the Accl site in GM at position 1429 became unique. All further constructs which contained the ERGIC-53 lumenal domain were generated by PCR using 3'-primers with a terminal XbaI restriction site. This allowed direct cloning of the PCR-amplified sequen.....
Document: For further mutagenesis, GM was cloned as a ClaI/XbaI fragment into AccI/XbaI cut pECE. The resulting plasmid was designated pECE*. This cloning step eliminated the single AccI site in pECE so that the Accl site in GM at position 1429 became unique. All further constructs which contained the ERGIC-53 lumenal domain were generated by PCR using 3'-primers with a terminal XbaI restriction site. This allowed direct cloning of the PCR-amplified sequences as AccI/XbaI fragments into pECE* that were then confirmed by sequencing.
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