Genomics and Proteomics of Mycobacteriophage Patience, an Accidental Tourist in the Mycobacterium Neighborhood

Pope, Welkin H.; Jacobs-Sera, Deborah; Russell, Daniel A.; Rubin, Daniel H. F.; Kajee, Afsana; Msibi, Zama N. P.; Larsen, Michelle H.; Jacobs, William R.; Lawrence, Jeffrey G.; Hendrix, Roger W.; Hatfull, Graham F.

Relationship of Patience to other mycobacteriophages. The Patience genome is not closely related to other phage genomes, although dot plot analysis shows weak similarity to cluster H phages (Fig. 2) . The related segments span only 14%, 8%, and 6% of genome lengths with Barnyard, Predator, and Konstantine genomes, respectively (Fig. 2) , and the closest matching segment of Patience and Barnyard is a 1,091-bp region (73% nucleotide identity) corresponding to the capsid subunit genes. Alignment of the genome maps of Patience, Barnyard, Konstantine, and Predator in Phamerator shows their architectural relationships (Fig. 3) , and although the nucleotide sequence similarity is minimal, 37% of Patience genes have homologues in cluster H phages (Fig. 3) . However, more than half of the Patience ORFs are orphams (genes with no close mycobacteriophage relatives [ Fig. 3 ; Table 1 ]).