Author: Yen, Wei-Chen; Wu, Yi-Hsuan; Wu, Chih-Ching; Lin, Hsin-Ru; Stern, Arnold; Chen, Shih-Hsiang; Shu, Jwu-Ching; Tsun-Yee Chiu, Daniel
Title: Impaired inflammasome activation and bacterial clearance in G6PD deficiency due to defective NOX/p38 MAPK/AP-1 redox signaling Document date: 2019_11_2
ID: 6fw4thkq_15
Snippet: THP-1 cells were cultured in the presence of 150 ng/ml PMA to allow for differentiation into macrophage-like cells. After incubation for 24 h, the cells were incubated with fresh RPMI 1640 for another 24 h, followed by 0.1 μg/ml LPS treatment and 20 μM nigericin treatment. The cell lysate and supernatant were collected for the indicated experiments......
Document: THP-1 cells were cultured in the presence of 150 ng/ml PMA to allow for differentiation into macrophage-like cells. After incubation for 24 h, the cells were incubated with fresh RPMI 1640 for another 24 h, followed by 0.1 μg/ml LPS treatment and 20 μM nigericin treatment. The cell lysate and supernatant were collected for the indicated experiments.
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