Title: 2017 ACVIM Forum Research Abstract Program Document date: 2017_6_15
ID: ri2w5iby_12
Snippet: A GWAS was performed with genomic DNA using a custom single nucleotide polymorphism (SNP) genotyping array containing 185,850 markers. Results were analyzed by calculating the odds ratio and P value for each SNP. A gene of interest was identified and overlapping primer pairs were designed to incorporate exon segments between 300-400 base pairs in length. Polymerase chain reactions (PCR) were performed using a touchdown program. Sanger sequencing .....
Document: A GWAS was performed with genomic DNA using a custom single nucleotide polymorphism (SNP) genotyping array containing 185,850 markers. Results were analyzed by calculating the odds ratio and P value for each SNP. A gene of interest was identified and overlapping primer pairs were designed to incorporate exon segments between 300-400 base pairs in length. Polymerase chain reactions (PCR) were performed using a touchdown program. Sanger sequencing was performed on a total of 20 samples (10 DMVD, 10 controls). Sequence alignments were performed using Clustal Omega software.
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