Author: Liu, Chunxi; Liu, Tingxian; Yu, Xiaoyue; Gu, Yizhu
Title: A preliminary study on the interaction between Asn-Gly-Arg (NGR)-modified multifunctional nanoparticles and vascular epithelial cells Document date: 2017_4_1
ID: xio0g203_25
Snippet: To further study the effect of cholesterol depletion on the interaction between the TPIC and CAV1, cells were pre-treated with MβCD and incubated with TPIC as previously mentioned. After being fixed, cells were incubated with a DyLight 488-labelled anti-human CAV1 monoclonal antibody (1:200 dilution) at 4 1C overnight. After extensive washing, the cells were stained by Hoechst33342, and the glass bottom dishes were examined under a confocal scan.....
Document: To further study the effect of cholesterol depletion on the interaction between the TPIC and CAV1, cells were pre-treated with MβCD and incubated with TPIC as previously mentioned. After being fixed, cells were incubated with a DyLight 488-labelled anti-human CAV1 monoclonal antibody (1:200 dilution) at 4 1C overnight. After extensive washing, the cells were stained by Hoechst33342, and the glass bottom dishes were examined under a confocal scanning microscope.
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