Author: Schlub, Timothy E; Buchmann, Jan P; Holmes, Edward C
Title: A Simple Method to Detect Candidate Overlapping Genes in Viruses Using Single Genome Sequences Document date: 2018_8_7
ID: yiqdsf9z_17
Snippet: The largest detected ORF was 708 codons long and located within Nhumirim virus, a positive-sense flavivirus recently isolated from mosquitoes in Brazil (Pauvolid-Correa et al. 2015) . Unexpectedly, this ORF is coded on a reverse complementary reading frame (Àc1), which means that unlike the other proteins in this virus, transcription must occur from a negative-sense RNA template. This finding invites further investigation of the potential mechan.....
Document: The largest detected ORF was 708 codons long and located within Nhumirim virus, a positive-sense flavivirus recently isolated from mosquitoes in Brazil (Pauvolid-Correa et al. 2015) . Unexpectedly, this ORF is coded on a reverse complementary reading frame (Àc1), which means that unlike the other proteins in this virus, transcription must occur from a negative-sense RNA template. This finding invites further investigation of the potential mechanisms by which transcription of reverse complementary reading frames might occur in positive-sense RNA viruses. In addition, the 26th codon in this ORF is a methionine (a common start codon) suggesting that a large component of the 708 codons may be transcribed.
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