Selected article for: "genomic dna and PCR product"
Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans
  • Document date: 1997_6_16
    • ID: pwgj97kz_32
    Snippet: Bases 1-278 of the ␣2,3 SAT (O) DNA were PCR amplified from T47D and MTSV1-7 genomic DNA prepared as described previously (D'Souza et al., 1993) , using the following oligonucleotides: 5ЈGAATTCGAA-TTCGGACTGCGAAGATG and 5ЈAAGCTTAAGCTTAAGAGCGCG-TTCTGGGC. The PCR product was cloned into the HindIII/EcoRI sites of pBluescript and sequenced using the ABI PRISM automated sequencing method (Perkin-Elmer Corp., Norwalk, CT), for comparison with the â.....
    Document: Bases 1-278 of the ␣2,3 SAT (O) DNA were PCR amplified from T47D and MTSV1-7 genomic DNA prepared as described previously (D'Souza et al., 1993) , using the following oligonucleotides: 5ЈGAATTCGAA-TTCGGACTGCGAAGATG and 5ЈAAGCTTAAGCTTAAGAGCGCG-TTCTGGGC. The PCR product was cloned into the HindIII/EcoRI sites of pBluescript and sequenced using the ABI PRISM automated sequencing method (Perkin-Elmer Corp., Norwalk, CT), for comparison with the ␣2,3 SAT-expressing plasmid.

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