Selected article for: "genome sequence and protein sequence"
Author: Paquin, Ashley; Onabajo, Olusegun O.; Tang, Wei; Prokunina-Olsson, Ludmila
Title: Comparative Functional Analysis of 12 Mammalian IFN-?4 Orthologs
  • Document date: 2016_1_1
    • ID: sqxrwgif_13
    Snippet: The expression constructs (human IFN-l4-Halo and control-Halo) based on the pFC14A vector (Promega) have previously been described (Prokunina-Olsson and others 2013). The control-Halo construct generates a Halo-tag protein with a size of 33 kDa, and the IFN-l4-Halo construct generates the IFN-l4-Halo protein (53 kDa) consisting of the IFN-l4 protein (20 kDa) C-terminally fused with the Halo-tag protein (33 kDa). For the animal expression construc.....
    Document: The expression constructs (human IFN-l4-Halo and control-Halo) based on the pFC14A vector (Promega) have previously been described (Prokunina-Olsson and others 2013). The control-Halo construct generates a Halo-tag protein with a size of 33 kDa, and the IFN-l4-Halo construct generates the IFN-l4-Halo protein (53 kDa) consisting of the IFN-l4 protein (20 kDa) C-terminally fused with the Halo-tag protein (33 kDa). For the animal expression constructs, fulllength open reading frames (ORFs) were predicted from DNA sequences available in the UCSC Genome browser based on similarity to human IFN-l4 protein sequence.

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