Author: Oh, Seo-ho; Kim Cho, Young-Saeng; Lee, Ho-Bin; Lee, Sang-Mok; Kim, Whee-Soo; Hong, Liang; Cho, Chong-Su; Choi, Yun-Jaie; Kang, Sang-Kee
Title: Enhancement of antigen-specific humoral immune responses and protein solubility through conjugation of bacterial flagellin, Vibrio vulnificus FlaB, to the N-terminus of porcine epidemic diarrhea virus surface protein antigen S0 Document date: 2019_11_5
ID: q4p77ukw_8
Snippet: The nucleotide sequence of the spike protein from the PEDV K14JB01 strain (GenBank accession No. KJ623926) was used as a reference to synthesize a partial protein from the S0 (amino acids 25-229) gene. The whole genome sequence from Vibrio vulnificus YJ016 (GenBank accession No. BA000037) was used as a reference to synthesize FlaB. Each sequence was codon-optimized using the table of codon usage in E. coli before synthesis (Genescript, USA; Bione.....
Document: The nucleotide sequence of the spike protein from the PEDV K14JB01 strain (GenBank accession No. KJ623926) was used as a reference to synthesize a partial protein from the S0 (amino acids 25-229) gene. The whole genome sequence from Vibrio vulnificus YJ016 (GenBank accession No. BA000037) was used as a reference to synthesize FlaB. Each sequence was codon-optimized using the table of codon usage in E. coli before synthesis (Genescript, USA; Bioneer, Korea) and PCR-amplified using various primer pairs provided in Table 1 .
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