Author: Imai-Matsushima, Aki; Martin-Sancho, Laura; Karlas, Alexander; Imai, Seiichiro; Zoranovic, Tamara; Hocke, Andreas C.; Mollenkopf, Hans-Joachim; Berger, Hilmar; Meyer, Thomas F.
Title: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Document date: 2018_6_22
ID: vopbw1tl_34
Snippet: To facilitate downstream applications, we optimized delivery of siRNA to achieve comparable knockdown efficiencies for human DAECs and A549 cells (Fig. 7A) and assessed whether this technology could be used to determine the impact of a given knockdown on viral replication. Knockdown of the influenza virus factor nucleoprotein (NP) resulted in reduced replication of the A/WSN/1933 (H1N1) strain at various MOIs ( Fig. 7B and C) , demonstrating the .....
Document: To facilitate downstream applications, we optimized delivery of siRNA to achieve comparable knockdown efficiencies for human DAECs and A549 cells (Fig. 7A) and assessed whether this technology could be used to determine the impact of a given knockdown on viral replication. Knockdown of the influenza virus factor nucleoprotein (NP) resulted in reduced replication of the A/WSN/1933 (H1N1) strain at various MOIs ( Fig. 7B and C) , demonstrating the potential of our model for infection biology and other downstream applications.
Search related documents:
Co phrase search for related documents- H1N1 strain and influenza virus factor: 1
- H1N1 strain and reduce replication: 1
- H1N1 strain and viral replication: 1, 2, 3, 4, 5, 6, 7
- infection biology and influenza virus: 1, 2, 3, 4, 5
- infection biology and viral replication: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15
- influenza virus and reduce replication: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- influenza virus and siRNA delivery: 1, 2, 3, 4, 5, 6, 7, 8, 9
- influenza virus and viral replication: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78
- influenza virus factor and reduce replication: 1
- influenza virus factor and viral replication: 1, 2, 3, 4, 5
- knockdown efficiency and siRNA delivery: 1
- reduce replication and viral replication: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72
- siRNA delivery and viral replication: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date