Author: Devon Chandler-Brown; Anna M. Bueno; Oguzhan Atay; David S. Tsao
Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Document date: 2020_4_10
ID: eui41zyg_2
Snippet: The backbone of COVID-19 diagnosis has been quantitative polymerase chain reaction (qPCR) tests. The limited number of qPCR machines and the limitations of qPCR protocols, which do not have safe stopping points or a readily automatable process, have hampered large-scale testing. Furthermore, the sensitivity of qPCR requires specific RNA extraction reagents that are currently in short supply. Other diagnostics for COVID-19 include blood-based immu.....
Document: The backbone of COVID-19 diagnosis has been quantitative polymerase chain reaction (qPCR) tests. The limited number of qPCR machines and the limitations of qPCR protocols, which do not have safe stopping points or a readily automatable process, have hampered large-scale testing. Furthermore, the sensitivity of qPCR requires specific RNA extraction reagents that are currently in short supply. Other diagnostics for COVID-19 include blood-based immunoassays, which are often less accurate than molecular assays and indirectly detects immune response to COVID-19 only after symptoms appear, and CRISPR-based assays that are still in development [7] .
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