Author: Alagaili, Abdulaziz N.; Briese, Thomas; Amor, Nabil M.S.; Mohammed, Osama B.; Lipkin, W. Ian
Title: Waterpipe smoking as a public health risk: Potential risk for transmission of MERS-CoV Document date: 2018_5_3
ID: q7ux2r53_11
Snippet: A QiaCube robot (Qiagen) was used for total nucleic acid extraction from the swabs using Cador Reagent kits (Qiagen). Real time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was applied using a OneStep Real-Time qPCR buffer (Invitro-gen, Life Technologies). Real time PCR was performed on BioRad CFX theromcyclers (BioRad). Primer and probes upstream of E gene (upE) were as described by Corman et al. (2012a,b) and synthesiz.....
Document: A QiaCube robot (Qiagen) was used for total nucleic acid extraction from the swabs using Cador Reagent kits (Qiagen). Real time quantitative reverse transcription polymerase chain reaction (RT-qPCR) was applied using a OneStep Real-Time qPCR buffer (Invitro-gen, Life Technologies). Real time PCR was performed on BioRad CFX theromcyclers (BioRad). Primer and probes upstream of E gene (upE) were as described by Corman et al. (2012a,b) and synthesized by MWG-Biotech (Germany).
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