Selected article for: "mannosidase II antiserum and Rabbit mannosidase II antiserum"
Author: Whitehouse, Caroline; Burchell, Joy; Gschmeissner, Stephen; Brockhausen, Inka; Lloyd, Kenneth O.; Taylor-Papadimitriou, Joyce
Title: A Transfected Sialyltransferase That Is Elevated in Breast Cancer and Localizes to the medial/trans-Golgi Apparatus Inhibits the Development of core-2–based O-Glycans
  • Document date: 1997_6_16
    • ID: pwgj97kz_25
    Snippet: Cells were grown on glass coverslips, washed with PBS, and fixed with 4% paraformaldehyde for 15 min. Cells were permeabilized with 0.1% Triton for 5 min, and then nonspecific binding was blocked with 10% FCS/PBS for 30 min. The cells were then incubated with the 9E10 mAb to the myc epitope (10 g/ml) or TEX-1, a rabbit antiserum which recognizes mannosidase II (diluted 1:50) (Slusarewicz, 1994) , and binding was detected with FITC-conjugated goat.....
    Document: Cells were grown on glass coverslips, washed with PBS, and fixed with 4% paraformaldehyde for 15 min. Cells were permeabilized with 0.1% Triton for 5 min, and then nonspecific binding was blocked with 10% FCS/PBS for 30 min. The cells were then incubated with the 9E10 mAb to the myc epitope (10 g/ml) or TEX-1, a rabbit antiserum which recognizes mannosidase II (diluted 1:50) (Slusarewicz, 1994) , and binding was detected with FITC-conjugated goat anti-mouse (diluted 1:40; Dako) or rhodaminelabeled swine anti-rabbit (diluted 1:40; Dako) secondary antibodies.

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