Author: TERAMICHI, Takurou; FUKUSHI, Shuetsu; HACHIYA, Yuma; MELAKU, Simenew Keskes; OGUMA, Keisuke; SENTSUI, Hiroshi
Title: Evaluation of serological assays available in a biosafety level 2 laboratory and their application for survey of Middle East respiratory syndrome coronavirus among livestock in Ethiopia Document date: 2019_11_5
ID: vijh6x1l_15
Snippet: According to the results of the previous study, antibody titers of ≥16 are treated as positive in neutralization test using VSV-MERS/GFP. In dromedary camels and cattle, 31 out of 38 and 1 out of 15, respectively, were MERS antibody positive. Goats and sheep were all MERS antibody negative (Table l) . Moreover, in 15 camels, the antibody titer was 16 or 32; in 7, it was 64 or 128; and in 9 and control positive rabbit serum, it was ≥256. The a.....
Document: According to the results of the previous study, antibody titers of ≥16 are treated as positive in neutralization test using VSV-MERS/GFP. In dromedary camels and cattle, 31 out of 38 and 1 out of 15, respectively, were MERS antibody positive. Goats and sheep were all MERS antibody negative (Table l) . Moreover, in 15 camels, the antibody titer was 16 or 32; in 7, it was 64 or 128; and in 9 and control positive rabbit serum, it was ≥256. The antibody titer and the positive rate of antibody against MERS-CoV increased with the age of the camels. The antibody titer in one cow was 64. All sera collected from animals on the attached farm of Nihon University were negative.
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