Selected article for: "PCR reaction and quantitative PCR reaction"
Author: FAN, Wen-Sheng; LI, He-Ming; HE, Yi-Ning; TANG, Ning; ZHANG, Li-Hua; WANG, Hai-Yong; ZHONG, Lian; CHEN, Jian-Cai; WEI, Tian-Chao; HUANG, Teng; MO, Mei-Lan; WEI, Ping
Title: Immune protection conferred by three commonly used commercial live attenuated vaccines against the prevalent local strains of avian infectious bronchitis virus in southern China
  • Document date: 2018_7_18
    • ID: ty3cxcdt_15
    Snippet: Viral RNA was extracted from trachea and kidney samples of each bird at 5 dpc using EasyPure RNA Purification Kit (TransGen Biotech, Beijing, China). First-strand cDNA was synthesized using random hexamers (TaKaRa, Tokyo, Japan). The IBV viral loads in trachea and kidney samples were detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) targeting the nucleocapsid (N) gene according to our previous description [8] ......
    Document: Viral RNA was extracted from trachea and kidney samples of each bird at 5 dpc using EasyPure RNA Purification Kit (TransGen Biotech, Beijing, China). First-strand cDNA was synthesized using random hexamers (TaKaRa, Tokyo, Japan). The IBV viral loads in trachea and kidney samples were detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) targeting the nucleocapsid (N) gene according to our previous description [8] .

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