Author: Chen, Wenbing; Li, Shousong; Shao, Biying; Zheng, Teng; Jiang, Shuxun; Huang, Xiaorong; Cai, Kaizhen; Zhang, Zhideng
Title: Preliminary Study on Detecting the SARS-CoV Specific Target cDNA Fragments by Multiplex PCR Document date: 2016_11_28
ID: r2upa4o0_6
Snippet: Using relevant primer pairs listed in Table 1 , three combinations of any two target fragments, 121 & 182 bp, 121 & 302 bp, and 182 & 302 bp, were amplified by duplex PCR in simulated positive samples (Lanes 9, 11, and 13 in Figure 1 ). No cDNA fragment band was found in the relevant negative controls with ddH 2 O (Lanes 8, 10, and 12 in Figure 1 )......
Document: Using relevant primer pairs listed in Table 1 , three combinations of any two target fragments, 121 & 182 bp, 121 & 302 bp, and 182 & 302 bp, were amplified by duplex PCR in simulated positive samples (Lanes 9, 11, and 13 in Figure 1 ). No cDNA fragment band was found in the relevant negative controls with ddH 2 O (Lanes 8, 10, and 12 in Figure 1 ).
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