Concerted 2-5A-Mediated mRNA Decay and Transcription Reprogram Protein Synthesis in dsRNA Response

Sneha Rath; Eliza Prangley; Jesse Donovan; Kaitlin Demarest; Yigal Meir; Ned Wingreen; Alexei Korennykh

For decay rates, data points from three biological replicates were plotted together. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint k cat /(k off +k cat /k on ) ~ k on . The specificity ratio S1/S2 is simplified to k on 1 /k on 2 . S1/S2 no longer depends on the binding affinity (K m ) or the catalytic activity (k cat ) and depends only on the association rate constant (k on ). For similar substrates such as different mRNAs k on will depend primarily on the hydrodynamic radius and should be similar for most mRNAs. If association is mediated by the ribosome, k on for different mRNAs could be determined by ribosome-mRNA or ribosome-RNase L association. . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint Figure S2 . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint A B . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/484675 doi: bioRxiv preprint