Selected article for: "antibody spike and room temperature"
Author: Rogers, J.; Schoepp, R.J.; Schröder, O.; Clements, T.L.; Holland, T.F.; Li, J.Q.; Li, J.; Lewis, L.M.; Dirmeier, R.P.; Frey, G.J.; Tan, X.; Wong, K.; Woodnutt, G.; Keller, M.; Reed, D.S.; Kimmel, B.E.; Tozer, E.C.
Title: Rapid discovery and optimization of therapeutic antibodies against emerging infectious diseases
  • Document date: 2008_5_13
    • ID: xkx56h0o_21
    Snippet: SARS-CoV spike protein (Protein Sciences, Lexington, KY, USA) was labeled with biotin using the Biotin Protein Labeling kit (Roche Diagnostics, Penzberg, Germany). For testing Fabs and IgGs, 4 nM (final concentration) spike protein was added to various concentrations of Fabs or IgGs in 250 ml PBS. The spike protein -antibody solution was incubated at room temperature for 45 min and then incubated at 48C overnight......
    Document: SARS-CoV spike protein (Protein Sciences, Lexington, KY, USA) was labeled with biotin using the Biotin Protein Labeling kit (Roche Diagnostics, Penzberg, Germany). For testing Fabs and IgGs, 4 nM (final concentration) spike protein was added to various concentrations of Fabs or IgGs in 250 ml PBS. The spike protein -antibody solution was incubated at room temperature for 45 min and then incubated at 48C overnight.

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