Author: Stewart, Meredith E.; Roy, Polly
Title: Structure-based identification of functional residues in the nucleoside-2'-O-methylase domain of Bluetongue virus VP4 capping enzyme Document date: 2015_2_24
ID: vzel6r43_8
Snippet: Site-directed mutagenesis as previously described [37] was used to introduce site-specific mutations into the exact copy of BTV-1 S4 (pUCBTV1T7S4) and coding region of BTV-10 VP4 (pAcYM1VP4; GenBank: D00509). Single mutations were introduced at the specific residues of the aspartic acid position 265 of VP4 to either a glutamic acid (D265E) or valine (D265V) and asparagine 311, tyrosine Y334 and arginine 367 residues to an alanine (A) referred to .....
Document: Site-directed mutagenesis as previously described [37] was used to introduce site-specific mutations into the exact copy of BTV-1 S4 (pUCBTV1T7S4) and coding region of BTV-10 VP4 (pAcYM1VP4; GenBank: D00509). Single mutations were introduced at the specific residues of the aspartic acid position 265 of VP4 to either a glutamic acid (D265E) or valine (D265V) and asparagine 311, tyrosine Y334 and arginine 367 residues to an alanine (A) referred to as N311A, Y334A and R367A. A construct with the triple mutation, N311A/Y334/R367A (NYR) was also generated.
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