Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2 Document date: 2020_3_12
ID: 1qn5y4gc_17
Snippet: cDNA of SARS-CoV-2 was made using SuperScript IV Reverse Transcriptase (Invitrogen) following manufacturer's instructions with modifications. Briefly, 10 pmol of random hexamer was used as reverse transcription primer and reaction was performed as follow: 20 minutes at 25℃, 30 minutes at 55℃ and 10 minutes at 80℃......
Document: cDNA of SARS-CoV-2 was made using SuperScript IV Reverse Transcriptase (Invitrogen) following manufacturer's instructions with modifications. Briefly, 10 pmol of random hexamer was used as reverse transcription primer and reaction was performed as follow: 20 minutes at 25℃, 30 minutes at 55℃ and 10 minutes at 80℃.
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