Selected article for: "optimal temperature and reaction temperature"
Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
  • Document date: 2020_3_12
    • ID: 1qn5y4gc_24
    Snippet: Next, we checked if amplification is improved at lower temperature (65℃) because optimal reaction temperature of SuperScript IV Reverse Transcriptase is 50-55℃ and manufacturer recommend 65-72℃ for optimal performance of Bst3.0. Notably, "Nsp3_1-61" and "S_1-2-2" primer sets show improved threshold time (Tt_av = 8.92 and 8.49, respectively). The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.110.....
    Document: Next, we checked if amplification is improved at lower temperature (65℃) because optimal reaction temperature of SuperScript IV Reverse Transcriptase is 50-55℃ and manufacturer recommend 65-72℃ for optimal performance of Bst3.0. Notably, "Nsp3_1-61" and "S_1-2-2" primer sets show improved threshold time (Tt_av = 8.92 and 8.49, respectively). The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.03.09.983064 doi: bioRxiv preprint

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