Selected article for: "gene expression and productive infection"
Author: Diao, Lirong; Zhang, Bianhong; Xuan, Chenghao; Sun, Shaogang; Yang, Kai; Tang, Yujie; Qiao, Wentao; Chen, Qimin; Geng, Yunqi; Wang, Chen
Title: Activation of c-Jun N-terminal kinase (JNK) pathway by HSV-1 immediate early protein ICP0
  • Cord-id: 14bu5b1a
  • Document date: 2005_8_1
    • ID: 14bu5b1a
    Snippet: The immediate early protein ICP0 encoded by herpes simplex virus 1 (HSV-1) is believed to activate transcription and consequently productive infection. The precise mechanisms of ICP0-mediated transactivation are under intensive study. Here, we demonstrate that ICP0 can strongly activate AP-1 responsive genes specifically. This activation is inhibited by c-Jun (S73A), c-Jun (S63/73A), TAK1 (K63W), but not by p38 (AF), ERK1 (K71R), ERK2 (K52R) and TRAF6 (C85A/H87A). We further investigate the rele
    Document: The immediate early protein ICP0 encoded by herpes simplex virus 1 (HSV-1) is believed to activate transcription and consequently productive infection. The precise mechanisms of ICP0-mediated transactivation are under intensive study. Here, we demonstrate that ICP0 can strongly activate AP-1 responsive genes specifically. This activation is inhibited by c-Jun (S73A), c-Jun (S63/73A), TAK1 (K63W), but not by p38 (AF), ERK1 (K71R), ERK2 (K52R) and TRAF6 (C85A/H87A). We further investigate the relevancy of ERK, JNK and p38 MAPK pathways using their respective inhibitors PD98059, SP600125 and SB202190. Only SP600125 significantly attenuates the AP-1 responsive gene activation by ICP0. Consistent with these, the JNK is remarkably activated in response to ICP0, and this JNK activation is shown to be significantly attenuated by TAK1 (K63W). It turns out that ICP0 interacts specifically with TAK1 and stimulates its kinase activity. These findings reveal a new molecular mechanism ICP0 explores to regulate gene expression.

    Search related documents:
    Co phrase search for related documents
    • absence presence and activity modulate: 1, 2
    • absence presence and activity promote: 1, 2
    • absence presence and lps lipopolysaccharide: 1, 2, 3, 4, 5, 6
    • absence presence and luc reporter: 1
    • absence presence and luciferase activity: 1, 2, 3, 4, 5
    • absence presence and luciferase assay: 1
    • absence presence and luciferase reporter: 1, 2, 3, 4, 5
    • absence presence and luciferase reporter gene: 1, 2
    • absence presence and luciferase reporter plasmid: 1
    • absence presence and luciferase vector: 1
    • absence presence and lysis buffer: 1, 2, 3, 4, 5
    • activate show and luciferase activity: 1
    • activate show and luciferase reporter: 1