Selected article for: "internal control and Master mix"
Author: Daniel F Gudbjartsson; Agnar Helgason; Hakon Jonsson; Olafur T Magnusson; Pall Melsted; Gudmundur L Norddahl; Jona Saemundsdottir; Asgeir Sigurdsson; Patrick Sulem; Arna B Agustsdottir; Berglind Eiriksdottir; Run Fridriksdottir; Elisabet E Gardarsdottir; Gudmundur Georgsson; Olafia S Gretarsdottir; Kjartan R Gudmundsson; Thora R Gunnarsdottir; Arnaldur Gylfason; Hilma Holm; Brynjar O Jensson; Aslaug Jonasdottir; Frosti Jonsson; Kamilla S Josefsdottir; Thordur Kristjansson; Droplaug N Magnusdottir; Louise le Roux; Gudrun Sigmundsdottir; Gardar Sveinbjornsson; Kristin E Sveinsdottir; Maney Sveinsdottir; Emil A Thorarensen; Bjarni Thorbjornsson; Arthur Love; Gisli Masson; Ingileif Jonsdottir; Alma Moller; Thorolfur Gudnason; Karl G Kristinsson; Unnur Thorsteinsdottir; Kari Stefansson
Title: Early Spread of SARS-Cov-2 in the Icelandic Population
  • Document date: 2020_3_30
    • ID: bbmcenpy_14
    Snippet: Testing for SARS-CoV-2 was performed either at LUH or deCODE using similar quantitative real-time PCR (qRT-PCR) methods. TaqManâ„¢ Fast Virus 1-step Master Mix, 2019-nCoV Assay kits v1 and 2019-nCov control kits were obtained from Thermo Fisher. Assay mix A, B and C were prepared containing FAMâ„¢ dye labelled probes for the SARS-CoV-2 specific genes ORF1ab, S-protein and N-protein, respectively. In addition, each assay mix contained VICâ„¢ dye l.....
    Document: Testing for SARS-CoV-2 was performed either at LUH or deCODE using similar quantitative real-time PCR (qRT-PCR) methods. TaqMan™ Fast Virus 1-step Master Mix, 2019-nCoV Assay kits v1 and 2019-nCov control kits were obtained from Thermo Fisher. Assay mix A, B and C were prepared containing FAM™ dye labelled probes for the SARS-CoV-2 specific genes ORF1ab, S-protein and N-protein, respectively. In addition, each assay mix contained VIC™ dye labelled probes for human RNase P as internal control. Samples from 96-well RNA sample plate(s) were dispensed into three wells each in a 384 plate layout, in addition to three negative (no template) and three positive controls. Assay mix was added in a total reaction volume of 12.5 µL per sample. All sample aliquoting and mixing at deCODE was performed with an . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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