Author: Qi Liu; Amita Gupta; Ayse Okesli-Armlovich; Wenjie Qiao; Curt R. Fischer; Mark Smith; Jan E. Carette; Michael C. Bassik; Chaitan Khosla
Title: Enhancing the Antiviral Efficacy of RNA-Dependent RNA Polymerase Inhibition by Combination with Modulators of Pyrimidine Metabolism Document date: 2020_3_25
ID: 1zk64gsg_92
Snippet: Reactions were conducted at room temperature in 100 µL in Eppendorf tubes. Mixtures contained 20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2, 2.5 mM ATP, 5 mM substrates (cytidine, CPU and 5-F-CPU), and 2 µM UCK2. After gently mixing for 24 h on a rocking shaker, the reaction mixtures were heated for 3 minutes at 95 ºC to denature UCK2 enzyme. Formation of CPUMP and 5-F-CPUMP were confirmed by LC-MS/MS ( Figure S1 )......
Document: Reactions were conducted at room temperature in 100 µL in Eppendorf tubes. Mixtures contained 20 mM HEPES (pH 7.2), 100 mM KCl, 2 mM MgCl2, 2.5 mM ATP, 5 mM substrates (cytidine, CPU and 5-F-CPU), and 2 µM UCK2. After gently mixing for 24 h on a rocking shaker, the reaction mixtures were heated for 3 minutes at 95 ºC to denature UCK2 enzyme. Formation of CPUMP and 5-F-CPUMP were confirmed by LC-MS/MS ( Figure S1 ).
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