Evaluations of serological test in the diagnosis of 2019 novel coronavirus (SARS-CoV-2) infections during the COVID-19 outbreak

Dachuan Lin; Lei Liu; Mingxia Zhang; Yunlong Hu; Qianting Yang; Jiubiao Guo; Youchao Dai; Yuzhong Xu; Yi Cai; Xinchun Chen; Kaisong Huang; Zheng Zhang

is the (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10. 1101 /2020 To assess the specificity and sensitivity of the chemiluminescence immunoassay method developed 146 based on the recombinant nucleocapsid antigen, serum from 29 healthy individuals, 51 tuberculosis 147 patients and 79 confirmed SARS-CoV-2 patients were employed and tested. More than six-fold and 148 eight folds higher average RLU (relative light units) values were observed in the SARS-CoV-2 patients 149 group in the IgM testing compared to that of the healthy cohort and tuberculosis patients ( Figure 1A) . (Table 1 ). In contrast, using the IgG testing, we only detected two false-positive cases 160 from the control group, which is in line with the higher specificity for IgG (97.5%) compared to that of 161 the IgM testing (81.25%) as above described. CC-BY-NC 4.0 International license It is made available under a author/funder, who has granted medRxiv a license to display the preprint in perpetuity.