Author: Ali Gibran; Runzhen Zhao; Mo Zhang; Krishan G. Jain; Jianjun Chang; Satoshi Komatsu; Xiaohui Fang; Beiyun Zhou; Jiurong Liang; Dianhua Jiang; Mistuo Ikebe; Michael A Matthay; Hong-Long Ji
Title: Fibrinolytic niche is requested for alveolar type 2 cell-mediated alveologenesis and injury repair Document date: 2020_3_25
ID: 4h930ksd_71
Snippet: A6 and scrambled A6 (sA6) peptides were synthesized by Genscript. Stock solutions (2 mM) were prepared by dissolving peptides in water according to the manufacturer's instruction. For 3D matrigel cultures, sorted Plau -/-AT2 cells were preincubated with either A6 or sA6 peptide (1 µM) for 30 min at room temperature. Wt AT2 cells were treated with 30 ng/mL CD44-blocking antibody for 30 min at room temperature. The same concentrations of A6, sA6 p.....
Document: A6 and scrambled A6 (sA6) peptides were synthesized by Genscript. Stock solutions (2 mM) were prepared by dissolving peptides in water according to the manufacturer's instruction. For 3D matrigel cultures, sorted Plau -/-AT2 cells were preincubated with either A6 or sA6 peptide (1 µM) for 30 min at room temperature. Wt AT2 cells were treated with 30 ng/mL CD44-blocking antibody for 30 min at room temperature. The same concentrations of A6, sA6 peptides, and CD44-blocking antibody were added to both the matrigel/medium mix and the culture medium placed under the transwell inserts. The culture medium was changed every 48 h.
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