Selected article for: "Ct value and qrt pcr"
Author: Daniel J Butler; Christopher Mozsary; Cem Meydan; David C Danko; Jonathan Foox; Joel Rosiene; Alon Shaiber; Ebrahim Afshinnekoo; Matthew MacKay; Fritz J Sedlazeck; Nikolay A Ivanov; Maria A Sierra; Diana Pohle; Michael Zeitz; Vijendra Ramlall; Undina Gisladottir; Craig D Westover; Krista Ryon; Benjamin Young; Chandrima Bhattacharya; Phyllis Ruggiero; Bradley W Langhorst; Nathan A Tanner; Justyn Gawrys; Dmitry Meleshko; Dong Xu; Jenny Xiang; Angelika Iftner; Daniela Bezdan; John Sipley; Lin Cong; Arryn Craney; Priya Velu; Ari Melnick; Iman A Hajirasouliha; Thomas Iftner; Mirella Salvatore; Massimo Loda; Lars F Westblade; Shawn Levy; Melissa Cushing; Nicholas P Tatonetti; Marcin Imielinski; Hanna Rennert; Christopher Mason
Title: Host, Viral, and Environmental Transcriptome Profiles of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2)
  • Document date: 2020_4_20
    • ID: kyoa5gsf_45
    Snippet: Samples were annotated using qRT-PCR cycle threshold (Ct) value for SARS-CoV-2 primers. Subjects with Ct less than or equal to 18 were assigned "high viral load" label, Ct between 18 and 24 were assigned "medium viral load" and Ct between 24 and 40 were assigned "low viral load" classes, with anything above Ct of 40 was classified as negative. We also predicted a combined viral load score using Ct, GloMax QuantiFluor readout from LAMP experiments.....
    Document: Samples were annotated using qRT-PCR cycle threshold (Ct) value for SARS-CoV-2 primers. Subjects with Ct less than or equal to 18 were assigned "high viral load" label, Ct between 18 and 24 were assigned "medium viral load" and Ct between 24 and 40 were assigned "low viral load" classes, with anything above Ct of 40 was classified as negative. We also predicted a combined viral load score using Ct, GloMax QuantiFluor readout from LAMP experiments and fraction of SARS-CoV-2 matching NGS reads in a sample. For this score (40-Ct), (LAMP readout) and (log10(SARS-CoV-2 fraction + 1e-6)) were all normalized between zero and one individually, and summed together using a combination weight of 5 for Ct, 3 for LAMP and 2 for NGS.

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