Selected article for: "PCR reaction and TE buffer"
Author: Chenyu Li; David N. Debruyne; Julia Spencer; Vidushi Kapoor; Lily Y. Liu; Bo Zhou; Lucie Lee; Rounak Feigelman; Grayson Burdon; Jeffrey Liu; Alejandra Oliva; Adam Borcherding; Hongdong Tan; Alexander E. Urban; Guoying Liu; Zhitong Liu
Title: High sensitivity detection of coronavirus SARS-CoV-2 using multiplex PCR and a multiplex-PCR-based metagenomic method
  • Document date: 2020_3_14
    • ID: 0hxan9rw_34
    Snippet: 50ng of Universal Human Reference RNA was converted into cDNA using random primers and SuperScriptâ„¢ IV Reverse Transcriptase by following the supplier recommended method (Thermo Fisher Scientific, Cat# 18090050). After reverse transcription, cDNA was purified with 2.4X volume of magnetic beads, and washed twice with 70% ethanol. Finally, the purified cDNA was dissolved in 1X TE buffer and used per multiplex PCR reaction......
    Document: 50ng of Universal Human Reference RNA was converted into cDNA using random primers and SuperScriptâ„¢ IV Reverse Transcriptase by following the supplier recommended method (Thermo Fisher Scientific, Cat# 18090050). After reverse transcription, cDNA was purified with 2.4X volume of magnetic beads, and washed twice with 70% ethanol. Finally, the purified cDNA was dissolved in 1X TE buffer and used per multiplex PCR reaction.

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