Author: Wenbin Ji; Yibo Luo; Ejaz Ahmad; Song-Tao Liu
Title: Coordination between discrete Mitotic Arrest Deficient 1 (MAD1) domains is required for efficient mitotic checkpoint signaling Document date: 2017_11_1
ID: i4yquw4k_15
Snippet: Next a mCherry-Mis12-MAD1 CTD4E phosphomimic mutant was prepared and surprisingly found to be also defective in maintaining the mitotic arrest (Fig. S4b) . We reasoned that for a functional mitotic checkpoint in cells one or more of the four residues identified through in vitro kinase assays could not tolerate mutations......
Document: Next a mCherry-Mis12-MAD1 CTD4E phosphomimic mutant was prepared and surprisingly found to be also defective in maintaining the mitotic arrest (Fig. S4b) . We reasoned that for a functional mitotic checkpoint in cells one or more of the four residues identified through in vitro kinase assays could not tolerate mutations.
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