Author: Mahesh K R Kalikiri; Mohammad Rubayet Hasan; Faheem Mirza; Thabisile Xaba; Patrick Tang; Stephan Lorenz
Title: High-throughput extraction of SARS-CoV-2 RNA from nasopharyngeal swabs using solid-phase reverse immobilization beads Document date: 2020_4_11
ID: bx2xspbe_19
Snippet: Our results show that the addition of carrier RNA is a double-edged sword. While the addition increases the extraction efficiency and consistency, we hypothesized that an excess of carrier RNA may inhibit the reverse transcription. Indeed, we observed a small negative effect of carrier RNA on the detection of a transcript when is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity......
Document: Our results show that the addition of carrier RNA is a double-edged sword. While the addition increases the extraction efficiency and consistency, we hypothesized that an excess of carrier RNA may inhibit the reverse transcription. Indeed, we observed a small negative effect of carrier RNA on the detection of a transcript when is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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