Author: David E. Ochayon; Ayad Ali; Pablo C. Alarcon; Durga Krishnamurthy; Leah C. Kottyan; Michael Borchers; Stephen N. Waggoner
Title: Interleukin-33 promotes type 1 cytokine expression via p38 MAPK in human natural killer cells Document date: 2019_9_20
ID: iuhhuy53_27
Snippet: Peripheral blood mononuclear (PBMCs) cells were isolated on a ficollhystopaque (GE, Marlborough, MA) gradient, and NK cells were enriched by negative selection using the NK-cell isolation kit immune magnetic beads per manufacturer's protocol (Miltentyi Biotec, San Diego, CA). Enriched cells were >90% CD56 + CD3 neg NK cells as determined by flow cytometry......
Document: Peripheral blood mononuclear (PBMCs) cells were isolated on a ficollhystopaque (GE, Marlborough, MA) gradient, and NK cells were enriched by negative selection using the NK-cell isolation kit immune magnetic beads per manufacturer's protocol (Miltentyi Biotec, San Diego, CA). Enriched cells were >90% CD56 + CD3 neg NK cells as determined by flow cytometry.
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