Author: Chin-Yi Chu; Xing Qiu; Matthew N. McCall; Lu Wang; Anthony Corbett; Jeanne Holden-Wiltse; Christopher Slaunwhite; Qian Wang; Christopher Anderson; Alex Grier; Steven R. Gill; Gloria S. Pryhuber; Ann R. Falsey; David J. Topham; Mary T. Caserta; Edward E. Walsh; Thomas J Mariani
Title: Insufficiency in airway interferon activation defines clinical severity to infant RSV infection Document date: 2019_5_20
ID: bx49tbui_48
Snippet: Quantitative real-time polymerase chain reaction (qPCR) was performed using aliquots of RNA samples obtained for sequencing. cDNA was generated from approximately 250 ng RNA using the iScript cDNA Synthesis Kit (BioRad, Hercules, CA) according to manufacturer's recommendations. PCR was performed using gene-specific primer sets Gene expression levels were calculated relative to PPIA (cyclophilin A) using the ddCT method......
Document: Quantitative real-time polymerase chain reaction (qPCR) was performed using aliquots of RNA samples obtained for sequencing. cDNA was generated from approximately 250 ng RNA using the iScript cDNA Synthesis Kit (BioRad, Hercules, CA) according to manufacturer's recommendations. PCR was performed using gene-specific primer sets Gene expression levels were calculated relative to PPIA (cyclophilin A) using the ddCT method.
Search related documents:
Co phrase search for related documents- gene specific primer set and real time: 1
- primer set and qPCR real time polymerase chain reaction: 1, 2, 3
- primer set and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39
- primer set and real time polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9
- primer set and real time polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9
- primer set and RNA sample: 1, 2, 3, 4, 5, 6, 7, 8, 9
- primer set and Synthesis kit: 1
- qPCR real time polymerase chain reaction and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- qPCR real time polymerase chain reaction and real time polymerase: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- qPCR real time polymerase chain reaction and real time polymerase chain reaction: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- qPCR real time polymerase chain reaction and RNA sample: 1
- real time and RNA sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56
- real time and sequencing obtain: 1, 2
- real time and Synthesis kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- real time polymerase and RNA sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- real time polymerase and sequencing obtain: 1
- real time polymerase chain reaction and RNA sample: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- real time polymerase chain reaction and sequencing obtain: 1
- RNA sample and sequencing obtain: 1
Co phrase search for related documents, hyperlinks ordered by date