Author: Chin-Yi Chu; Xing Qiu; Matthew N. McCall; Lu Wang; Anthony Corbett; Jeanne Holden-Wiltse; Christopher Slaunwhite; Qian Wang; Christopher Anderson; Alex Grier; Steven R. Gill; Gloria S. Pryhuber; Ann R. Falsey; David J. Topham; Mary T. Caserta; Edward E. Walsh; Thomas J Mariani
Title: Insufficiency in airway interferon activation defines clinical severity to infant RSV infection Document date: 2019_5_20
ID: bx49tbui_48
Snippet: Quantitative real-time polymerase chain reaction (qPCR) was performed using aliquots of RNA samples obtained for sequencing. cDNA was generated from approximately 250 ng RNA using the iScript cDNA Synthesis Kit (BioRad, Hercules, CA) according to manufacturer's recommendations. PCR was performed using gene-specific primer sets Gene expression levels were calculated relative to PPIA (cyclophilin A) using the ddCT method......
Document: Quantitative real-time polymerase chain reaction (qPCR) was performed using aliquots of RNA samples obtained for sequencing. cDNA was generated from approximately 250 ng RNA using the iScript cDNA Synthesis Kit (BioRad, Hercules, CA) according to manufacturer's recommendations. PCR was performed using gene-specific primer sets Gene expression levels were calculated relative to PPIA (cyclophilin A) using the ddCT method.
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