Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo Document date: 2018_4_6
ID: 1pbshnw9_38
Snippet: For cellular smRNA-seq from HEK293T cells, we observed lower levels of single nucleotide additions (4.0%) and higher levels of multiple non-templated 3' additions (3.3%), compared to AGO-bound miRNAs (8.4% and 1.1% respectively in human). (Fig. S1D) . These differences may indicate that short oligo-nucleotide additions, which in some cases can promote pre-miRNA processing, could in fact prevent proper AGO loading of the 3' mature miRNA. Collectiv.....
Document: For cellular smRNA-seq from HEK293T cells, we observed lower levels of single nucleotide additions (4.0%) and higher levels of multiple non-templated 3' additions (3.3%), compared to AGO-bound miRNAs (8.4% and 1.1% respectively in human). (Fig. S1D) . These differences may indicate that short oligo-nucleotide additions, which in some cases can promote pre-miRNA processing, could in fact prevent proper AGO loading of the 3' mature miRNA. Collectively, these data . CC-BY 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/294488 doi: bioRxiv preprint demonstrate that non-templated additions are widespread in both pre-miRNAs and mature miRNAs, and that extended tails are more frequent in pre-miRNAs compared to mature miRNAs.
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