An amphiphilic dendrimer as a light-activable immunological adjuvant for in situ cancer vaccination.

Wang, Yongchao; Gong, Ningqiang; Ma, Chi; Zhang, Yuxuan; Tan, Hong; Qing, Guangchao; Zhang, Jimei; Wang, Yufei; Wang, Jinjin; Chen, Shizhu; Li, Xianlei; Ni, Qiankun; Yuan, Yuan; Gan, Yaling; Chen, Junge; Li, Fangzhou; Zhang, Jinchao; Ou, Caiwen; Zhao, Yongxiang; Liu, Xiaoxuan; Liang, Xing-Jie

Immunological adjuvants are essential for successful cancer vaccination. However, traditional adjuvants have some limitations, such as lack of controllability and induction of systemic toxicity, which restrict their broad application. Here, we present a light-activable immunological adjuvant (LIA), which is composed of a hypoxia-responsive amphiphilic dendrimer nanoparticle loaded with chlorin e6. Under irradiation with near-infrared light, the LIA not only induces tumour cell lysis and tumour antigen release, but also promotes the structural transformation of 2-nitroimidazole containing dendrimer to 2-aminoimidazole containing dendrimer which can activate dendritic cells via the Toll-like receptor 7-mediated signaling pathway. The LIA efficiently inhibits both primary and abscopal tumour growth and induces strong antigen-specific immune memory effect to prevent tumour metastasis and recurrence in vivo. Furthermore, LIA localizes the immunological adjuvant effect at the tumour site. We demonstrate this light-activable immunological adjuvant offers a safe and potent platform for in situ cancer vaccination.