Author: Sanchita Bhadra; Miguel A. Saldaña; Hannah Grace Han; Grant L. Hughes; Andrew D. Ellington
Title: Multiplex logic processing isothermal diagnostic assays for an evolving virus Document date: 2018_9_23
ID: n5liudlg_18
Snippet: The complete multiplex assay system (4-Plex-LAMP-2GO) containing 21 degenerate LAMP primers and the bipartite signal processor (CAN3.2GO + N1N5.2GO) was tested using different amounts of a synthetic RNA mixture containing all four ZIKV templates (NS1, NS3, NS5, and CA). Assays containing only a few hundred copies of ZIKV RNA produced bright fluorescence that could be readily captured using unmodified smartphone camera ( Figure 6C ). In contrast, .....
Document: The complete multiplex assay system (4-Plex-LAMP-2GO) containing 21 degenerate LAMP primers and the bipartite signal processor (CAN3.2GO + N1N5.2GO) was tested using different amounts of a synthetic RNA mixture containing all four ZIKV templates (NS1, NS3, NS5, and CA). Assays containing only a few hundred copies of ZIKV RNA produced bright fluorescence that could be readily captured using unmodified smartphone camera ( Figure 6C ). In contrast, assays containing non-specific amplicons remained dark thereby allowing easy visual distinction from ZIKV-positive assays.
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