Author: Roni Ben-Ami; Agnes Klochendler; Matan Seidel; Tal Sido; Ori Gurel-Gurevich; Moran Yassour; Eran Meshorer; Gil Benedek; Irit Fogel; Esther Oiknine-Djian; Asaf Gertler; Zeev Rotstein; Bruno Lavi; Yuval Dor; Dana G Wolf; Maayan Salton; Yotam Drier
Title: Pooled RNA extraction and PCR assay for efficient SARS-CoV-2 detection Document date: 2020_4_22
ID: 5s03f0pp_13
Snippet: To further reduce the need to retest positive pools we have also tested a two-stage matrix pooling strategy [20, 21] , where n 2 samples are ordered in an n x n matrix. Each row and each column are pooled, resulting in 2n tests, (Figure 2) . Importantly, the positive samples were detected in both the row and the column pools at a similar cycle in all three tested matrices, suggesting the pooling scheme is robust......
Document: To further reduce the need to retest positive pools we have also tested a two-stage matrix pooling strategy [20, 21] , where n 2 samples are ordered in an n x n matrix. Each row and each column are pooled, resulting in 2n tests, (Figure 2) . Importantly, the positive samples were detected in both the row and the column pools at a similar cycle in all three tested matrices, suggesting the pooling scheme is robust.
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