Potential host range of multiple SARS-like coronaviruses and an improved ACE2-Fc variant that is potent against both SARS-CoV-2 and SARS-CoV-1

Yujun Li; Haimin Wang; Xiaojuan Tang; Danting Ma; Chengzhi Du; Yifei Wang; Hong Pan; Qing Zou; Jie Zheng; Liangde Xu; Michael Farzan; Guocai Zhong

Methods and associated references are provided below. 147 author/funder. All rights reserved. No reuse allowed without permission. RBD residues in less than 5 Ã… from ACE2 atoms and ACE2 residues in less than 5 Ã… from RBD atoms are shown. b, The sequences of the SARS-CoV-2 WHU01, a pangolin coronavirus (Pangolin-CoV), a bat coronavirus (Bat-CoV RaTG13), and the SARS-CoV-1 BJ01 are aligned, with residues different from the corresponding ones in SARS-CoV-2 highlighted in blue. The stars indicate RBD residues in less than 5 Ã… from ACE2 atoms. The yellow lines indicate the RBM region, and the red lines or arrows indicate disulfide linkages. N-linked glycosylation motifs are indicated in green. c, Sequences of ACE2 orthologs from the 17 indicated species are aligned, with only residues in less than 5 Ã… from RBD atoms shown here and aligned full-length protein sequences shown in the Extended Data Figure 1 . The numbering is based on human ACE2 protein, and the residues different from the corresponding ones in human ACE2 are highlighted in blue.