Selected article for: "PCR buffer and reaction mixture"
Author: lianhua dong; Junbo Zhou; Chunyan Niu; Quanyi Wang; Yang Pan; Xia Wang; Yongzhuo zhang; Jiayi yang; Manqing Liu; Yang zhao; Tao peng; Jie xie; yunhua gao; Di wang; yun zhao; Xinhua Dai; xiang fang
Title: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
  • Document date: 2020_3_18
    • ID: aa7slcnc_10
    Snippet: Three different commercial RT-qPCR kits (H&R from Shanghai Huirui Biotechnology Co., Ltd, BioGerm from Shanghai BioGerm Medical Biotechnology and Daan from Daan Gene Co., Ltd) were used for the detection. Briefly, a 25-μL reaction containing 7.5 μ L of PCR reaction buffer, 5 µL of primer and probe mixture and 5~11 μ L of RNA was prepared. Thermal cycling was performed at 50 °C for 15 min for reverse transcription, followed by 95°C for 5 min.....
    Document: Three different commercial RT-qPCR kits (H&R from Shanghai Huirui Biotechnology Co., Ltd, BioGerm from Shanghai BioGerm Medical Biotechnology and Daan from Daan Gene Co., Ltd) were used for the detection. Briefly, a 25-μL reaction containing 7.5 μ L of PCR reaction buffer, 5 µL of primer and probe mixture and 5~11 μ L of RNA was prepared. Thermal cycling was performed at 50 °C for 15 min for reverse transcription, followed by 95°C for 5 min and then 45 cycles of 95 °C for 10 s, 55 °C for 45 s in ABI 7500 RT-PCR thermocycler. Data analysis was performed using software of ABI 7500 RT-PCR thermocycler.

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