Reverse transcription loop-mediated isothermal amplification combined with nanoparticles-based biosensor for diagnosis of COVID-19

Xiong Zhu; Xiaoxia Wang; Limei Han; Ting Chen; Licheng Wang; Huan Li; Sha Li; Lvfen He; Xiaoying Fu; Shaojin Chen; Xing Mei; Hai Chen; Yi Wang

(which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037796 doi: medRxiv preprint (Figure 1 and 2) . Two target sequences, including F1ab and nucleoprotein gene (np), 114 were simultaneously amplified in an isothermal reaction, and detected in a test step. 115 We will expound the basic COVID-19 RT-LAMP principle, optimize the reaction 116 parameters (e.g., amplification temperature), and demonstrate its feasibility. product also severed as the template for next amplification by LF* (forward loop 130 primer), which was modified at the 5' end with hapten (Step 5). As a result, a 131 double-labeled detectable product (LF*/LB* product) was formed, and one end of the 132 LF*/LB* product was labeled with hapten, and the other end with biotin (Step 6, 7). 133 One hapten is assigned to one primer set, which provide the possibility for multiplex 134 LAMP detection. CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.