Author: Lucia Grenga; Fabrice Gallais; Olivier Pible; Jean-Charles Gaillard; Duarte Gouveia; Hélène Batina; Niza Bazaline; Sylvie Ruat; Karen Culotta; Guylaine Miotello; Stéphanie Debroas; Marie-Anne Roncato; Gérard Steinmetz; Charlotte Foissard; Anne Desplan; Béatrice Alpha-Bazin; Christine Almunia; Fabienne Gas; Laurent Bellanger; Jean Armengaud
Title: Shotgun proteomics of SARS-CoV-2 infected cells and its application to the optimisation of whole viral particle antigen production for vaccines Document date: 2020_4_17
ID: kd02dehk_22
Snippet: To understand the dynamics associated with SARS-CoV-2 infection and determine optimal conditions for whole-viral particle antigen production we infected Vero E6 cells with SARS-CoV-2 at two multiplicity of infection (MOI 0.01 and 0.001) and monitored the kinetics of the infection by means of tandem mass spectrometry over several days ( Figure 1 ). Overall, we identified 3220 Vero cell proteins and 6 SARS-CoV-2 proteins with 27388 and 94 unique pe.....
Document: To understand the dynamics associated with SARS-CoV-2 infection and determine optimal conditions for whole-viral particle antigen production we infected Vero E6 cells with SARS-CoV-2 at two multiplicity of infection (MOI 0.01 and 0.001) and monitored the kinetics of the infection by means of tandem mass spectrometry over several days ( Figure 1 ). Overall, we identified 3220 Vero cell proteins and 6 SARS-CoV-2 proteins with 27388 and 94 unique peptides (FDR below 1%), respectively (Table S1) was in accordance to notable cytopathic effect following intense virus replication observed here for each culture at this time-point, as previously reported [22] .
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