Selected article for: "hydrogen peroxide and phospho poly ub"
Author: Lyudmila Kovalchuke; Eugene V. Mosharov; Oren A. Levy; Lloyd A. Greene
Title: Stress-induced phospho-ubiquitin formation causes parkin degradation
  • Document date: 2018_12_5
    • ID: ceepyyxj_21
    Snippet: Having placed a portion of parkin loss from L-DOPA in the same pathway as L-DOPAinduced formation of phospho-Ub, we next asked whether, and to what extent, parkin interaction with phospho-Ub plays a role in its loss from other stressors. Given that hydrogen peroxide causes parkin loss (Fig. 2D ) and induces phospho-poly-Ub formation (Fig. 4D) , we first queried whether parkin loss from this oxidative stressor is also mediated by its interaction w.....
    Document: Having placed a portion of parkin loss from L-DOPA in the same pathway as L-DOPAinduced formation of phospho-Ub, we next asked whether, and to what extent, parkin interaction with phospho-Ub plays a role in its loss from other stressors. Given that hydrogen peroxide causes parkin loss (Fig. 2D ) and induces phospho-poly-Ub formation (Fig. 4D) , we first queried whether parkin loss from this oxidative stressor is also mediated by its interaction with phospho-Ub. To achieve this, we utilized neuronal PC12 cells moderately over-expressing either wildtype or various mutant forms of parkin. Indeed, while wild-type parkin levels decreased by half upon peroxide treatment (50.9 ± 4.3% parkin remaining; p = 0.001, N = 5), the H302A and K151E parkin mutants were nearly completely rescued from loss induced by this agent (K151E + peroxide: 96.2 ± 8.0% parkin remaining, N = 5, p = 0.97 vs. no treatment; H302A + peroxide:

    Search related documents:
    Co phrase search for related documents
    • hydrogen peroxide and oxidative stressor: 1
    • hydrogen peroxide and parkin loss: 1, 2, 3, 4, 5, 6, 7
    • hydrogen peroxide and parkin loss portion: 1
    • hydrogen peroxide and parkin mutant: 1, 2, 3, 4
    • hydrogen peroxide and peroxide treatment: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
    • loss role and parkin loss: 1, 2, 3, 4
    • loss role play and parkin loss: 1
    • oxidative stressor and parkin loss: 1