Selected article for: "ifn response and protease activity"
Author: Xufang Deng; Yafang Chen; Anna M. Mielech; Matthew Hackbart; Kristina R. Kesely; Robert C. Mettelman; Amornrat O’Brien; Mackenzie E. Chapman; Andrew D. Mesecar; Susan C. Baker
Title: Structure-Guided Mutagenesis Alters Deubiquitinating Activity and Attenuates Pathogenesis of a Murine Coronavirus
  • Document date: 2019_9_25
    • ID: l3qp0n9f_8
    Snippet: Recombinant MHV harboring PLP2-D1772A activates an earlier IFN response in bone 168 marrow-derived macrophages. Since the D1772A substitution did not impact protease activity, 169 we reasoned that we should be able to generate recombinant virus containing this substitution, 170 thereby allowing us to determine if the mutation has any effect on viral replication kinetics and 171 interferon antagonism in the context of the live virus. We engineered.....
    Document: Recombinant MHV harboring PLP2-D1772A activates an earlier IFN response in bone 168 marrow-derived macrophages. Since the D1772A substitution did not impact protease activity, 169 we reasoned that we should be able to generate recombinant virus containing this substitution, 170 thereby allowing us to determine if the mutation has any effect on viral replication kinetics and 171 interferon antagonism in the context of the live virus. We engineered the mutant virus via reverse 172 genetics (32), performed full genome sequencing to verify the genotype (2 nucleotide changes at 173 positions 5525 and 5526, resulting in D1772A substitution in the replicase polyprotein), and 174 All rights reserved. No reuse allowed without permission.

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