Selected article for: "cut value and inhibition percent"
Author: Lau, Susanna K. P.; He, Zirong; Tsang, Chi-Ching; Chan, Tony T. Y.; Luk, Hayes K. H.; Chan, Elaine; Li, Kenneth S. M.; Fung, Joshua; Chow, Franklin W. N.; Tam, Anthony R.; Chung, Tom W. H.; Wong, Sally C. Y.; Que, Tak-Lun; Fung, Kitty S. C.; Lung, David C.; Wu, Alan K. L.; Hung, Ivan F. N.; Teng, Jade L. L.; Wernery, Ulrich; Hui, Suk-Wai; Martelli, Paolo; Woo, Patrick C. Y.
Title: A Sensitive and Specific Competitive Enzyme-Linked Immunosorbent Assay for Serodiagnosis of COVID-19 in Animals
  • Cord-id: bvnkub6k
  • Document date: 2021_5_10
    • ID: bvnkub6k
    Snippet: In addition to human cases, cases of COVID-19 in captive animals and pets are increasingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inact
    Document: In addition to human cases, cases of COVID-19 in captive animals and pets are increasingly reported. This raises the concern for two-way COVID-19 transmission between humans and animals. Here, we developed a SARS-CoV-2 nucleocapsid protein-based competitive enzyme-linked immunosorbent assay (cELISA) for serodiagnosis of COVID-19 which can theoretically be used in virtually all kinds of animals. We used 187 serum samples from patients with/without COVID-19, laboratory animals immunized with inactive SARS-CoV-2 virions, COVID-19-negative animals, and animals seropositive to other betacoronaviruses. A cut-off percent inhibition value of 22.345% was determined and the analytical sensitivity and specificity were found to be 1:64–1:256 and 93.9%, respectively. Evaluation on its diagnostic performance using 155 serum samples from COVID-19-negative animals and COVID-19 human patients showed a diagnostic sensitivity and specificity of 80.8% and 100%, respectively. The cELISA can be incorporated into routine blood testing of farmed/captive animals for COVID-19 surveillance.

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